Goal: Image as many cells as possible in the mosquito tarsi while applying different chemosensory stimulants.
Mosquito leg is hydrophobic
Scales seriously occlude imaging
Cells are really close together
[360 image of tarsi]
Microscopy techniques considered:
Volumetric LFM (considered but didn't try)
2-photon (considered but didn't try)
I started this part of the project using imaging wells -- I started with Nunc Lab-Tek 4-well chambers, and also used Ibidi's very similar product. The important thing about the wells is that they have a glass coverslip bottom.
Short term tests
Test GC7 animals, test tarsi only --> get a KCl response using old imaging parameters